Supplemental Material

This article contains the following supporting material:

• Figure 1 - Deletion of both PTS1 and putative PTS2 motifs of PpPex8p impairs its targeting to peroxisomes. Wild-type and Δpex8 strains expressing GFP-Pex8ΔAKL-PTS2m were grown in YNM for 4 h, and visualized by fluorescence microscopy and Normarski optics. Scale bar : 2 μm.
• Figure 2 - An alternative method for protease-protection analysis. The P200 fraction (50 μg/reaction) of oleate-grown Δpex14 cells was incubated with proteinase K (20 μg/reaction) for various times, in the presence (+) or absence (-) of Triton X-100. Proteins were separated by SDS-PAGE and immunoblotted with the indicated antibodies. Note that PpPex8p is not in peroxisomes, which is the same conclusion as that from the experiment in Figure 7A, thereby establishing the equivalence of the two methods used to assess protease protection of peroxisomal proteins.