Supplemental Material

This article contains the following supporting material:

• Movie 1 - Chemotaxis of pi3k1/2-null cells pre-treated with 60 μM LY294002 for 30 min towards a capillary filled with 100 μM cAMP. Total time is 24 min, recorded at 1 frame per second. The field of observation is 358x269 μm.

• Movie 2 - supplement to Table II. pi3k-null.avi Chemotaxis of pi3k1/2-null cells towards a capillary filled with 100 uM cAMP. Total time is 17 min, recorded at 1 frame per second. The field of observation is 358x269 um.

• Movie 3 - supplement to figure 2. UniformLatA.avi Translocation of PHcrac-GFP in cells pre-treated for 5 min with 1 uM Latrunculin A (LatA). Cells are perfused with buffer for 30 s, with 1 uM cAMP for 90 s, followed by buffer for 30 s. Total time is 180 s, recorded at one frame per second.