Supplemental Materials

This article contains the following supporting material:

• Video 1 - 3-D reconstruction of 30 optical sections from a single MDCKT cell labeled basolaterally with Tfn (green) and apically with FcLR(5-22) (red). Only endosomes are shown. REs are specifically labeled by Tfn. Inset shows a close-up of supra-nuclear endosomes. Note adjacent apical and basolateral subdomains with overlap. 3-D construction produced and rotated in Improvision Volocity software. Display rate 15 fps. See also Fig. 1.
• Video 2 - 3-D reconstruction of 30 optical sections from 2 MDCK cells expressing LDLR. Cells were apically labeled with wheat germ agglutinin (green) and immunolabeled (after permeabilization) for LDLR (red). Exemplary RE is indicated by green arrow. Display rate 24 fps.