Supplemental Material

This article contains the following supporting material:

• Figure 1 - Incorporation and quenching of NBD-PC in SVs

• Figure 2 - Pma1p and Drs2p-containing membranes from 38C-shifted sec6-4 cells do not co-fractionate with TGN marker Kex2p.

• Figure 3 - Loss of Drs2p and Dnf3p reduces the TNBS-reactive PE pool in SV-enriched membranes