Supplemental Material

This article contains the following supporting material:

• Movie 1 - Abp1-GFP in wildtype strain YJC 3396. Video speed 25 frames/sec, and images collected at 5 frames/sec, so 5X real time. Colored dots indicate patches being tracked by the computer program.

• Movie 2 - Abp1-GFP in cap1Δ strain YJC 3920. Video speed 25 frames/sec, and images collected at 5 frames/sec, so 5X real time.

• Movie 3 - FM4-64 in wildtype strain YJC 2588. Video speed 5 frames/sec, and images collected at 1 frame/sec, so 5X real time.

• Movie 4 - FM4-64 in cap1Δ strain YJC 2538. Video speed 5 frames/sec, and images collected at 1 frame/sec, so 5X real time.

• Movie 5 - Ste2-GFP in wildtype strain YJC 2588. Video speed 25 frames/sec, and images collected at 5 frames/sec, so 5X real time.

• Movie 6 - Ste2-GFP in cap1Δ strain. YJC 2538. Video speed 25 frames/sec, and images collected at 5 frames/sec, so 5X real time.