Supplemental Material

This article contains the following supporting material:

• Figure 1 - kin-10/ CK2βƒnand kin-3/CK2α are co-expressed with pkd-2 in male-specific neurons.
  Confocal micrographs of Ppkd-2::DsRed2 (using the pkd-2 promoter to drive DsRed2 expression) and Pkin-3::GFP (using kin-3 promoter to drive GFP expression) (a, b) or Pkin-10::GFP (using kin-10 promoter to drive GFP expression) (c, d) double-labeled C.elegans males. In all figures, adult males are oriented anterior to posterior, with head pointing left (a, c) and tail (b, d) pointing right. While lov-1 and pkd-2 are expressed in identical subsets of male-specific neurons, the pkd-2 promoter is stronger and more suitable for coexpression and heterologous expression studies. kin-3 and kin-10 coexpress with pkd-2 in male-specific neurons both in adult male head (a, c) and tail (b, d). Note the presence of Pkin-3::GFP and Pkin-10::GFP in all 9 pairs of RnB neurons and the exclusion of Ppkd-2::DsRed2 in ray 6 neuron (R6B). Numbers indicate the corresponding rays. Labeled bar indicates length in micrometer (μm).

• Figure 2 - TAX-6 localizes to cilia.
  Confocal micrographs of the head (top) and tail (bottom) of a Ptax-6::TAX-6::GFP transgenic adult male. TAX-6::GFP is distributed throughout male sensory neurons in cell bodies (including nuclei), dendrites, axons, and cilia. Labeled bars indicate length in micrometer (μm).