Supplemental Material

This article contains the following supporting material:

• Figure 1 - Cellular localization of GFP-Cep55 N/C.
  HeLa cells were transfected with GFP-Cep55 N (amino acid 1-220) or GFP-Cep55 C (amino acid 220-464), fixed at 30 hours post-transfection and immuno-stained with anti-β-tubulin (red) antibody. DNA, blue. Bar, 5 μm.

• Movie 1 - Time-lapse movies of HeLa cells undergoing cytokinesis.
  Cells were transfected with siControl (Movie 1) or siCep55 (Movie 2) and imaged every 3 min starting from 30 hours post-transfection. DIC images were shown in the movies.
• Movie 2