Pericentromeric Heterochromatin Domains Are Maintained without Accumulation of HP1
Mol. Biol. Cell Mateos-Langerak et al.
18: 1464
Supplemental Material
This article contains the following supporting material:
Supplemental Figure 1A
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Spatial distribution of transfected truncated HP1, endogenous HP1 and DAPI stained heterochromatin domains
Cells transfected with HP1α- Δ (2-39)-flag (A-D) and HP1β-Δ (2-40)-flag (E-I) were fluorescently labeled 24 h after transfection. The anti-flag signal (first column) shows the transfected HP1α- Δ (2-39)-flag cells (A-D) and the transfected HP1β-Δ(2-40)-flag cells (E-I). The distribution of endogenous HP1 is shown in the second column; HP1α?after labeling with RaHP1α?(A and E) or with MaHP1α (B and F) HP1β after labeling with RaaHP1β?(C and G) or with MaHP1β (D and H) and HP1γ after labeling with MaHP1γ (I). The DAPI staining is shown in the right column (A-I).