Supplemental Material

This article contains the following supporting material:

• Figure 1 - Plant growth responses to D-glucose.

• Figure 2 - Semiquantitative RT-PCR profiling RNA encoding various G-protein elements in wild type and SGB1-1^D, sgb1-2 mutants.

• Table 1 - Primers used for cloning, DNA sequencing, PCR and RT PCR.

• Table 2 - Yeast strains used in this study.

• Table 3 - Constructs used in this paper.

• Movie 1 - Rapid movement of SGB1 labeled compartments in hypocotyls cells before the addition of 1% D-Glucose. SGB1 is partially localized to Golgi.

• Movie 2 - Rapid movement of SGB1 labeled compartments in hypocotyls cells 90 min after the addition 1% D-Glucose. D- Glucose caused an increase in SGB1 labeled Golgi.

• Movie 3 - Rapid movement of SGB1 labeled compartments in hypocotyls cells 120 min after the addition of 1% D-Glucose. D- Glucose caused an increase in SGB1 labeled Golgi and a reduction in smaller SGB1 labeled compartments.