Supplemental Material

This article contains the following supporting material:

• Figure 1 - Mitochondrial morphology in yeast F-box protein mutants. Wild type cells (WT) and yeast mutants lacking non-essential F-box protein-encoding genes AMN1 (YBR158w), COS111 (YBR203w), DIA2 (YOR080w), ELA1 (YNL230c), GRR1 (YJR090c), HRT3 (YLR097c), RCY1 (YJL204c), SKP2 (YNL311c), UFO1 (YML088w), YBR280c, YDR131c, YDR306c, YJL149w, YLR224w, YLR352w, and YMR258c were grown to logarithmic growth phase in YPD medium. Yeast mutants carrying essential F-box protein-encoding genes CDC4 (YFL009w), CFT13 (YMR094w), and MET30 (YIL046w) under control of a titratable promoter were grown in YPD medium containing doxycycline (+ Dox). Living cells expressing mitochondria-targeted GFP were analyzed by DIC and fluorescence microscopy, and merged images are shown.

• Figure 2 - Cells lacking Mfb1 contain normal vacuoles, ER and actin cytoskeleton. Vacuoles in living wild type and Δmfb1 cells were stained with 5-(and-6)-carboxy-2’,7’-dichlorofluorescin diacetate, and ER was stained with ER-targeted GFP. For staining of filamentous actin, cells were fixed and stained with rhodamine phalloidin. Samples were analyzed by fluorescence and DIC microscopy.