Supplemental Material

This article contains the following supporting material:

• Figure 1 - Stimulation of SPR-Hela cells with substance P or TPA does not change the basal membrane localization of Epsin, Eps15 or Dab2.
  SPR-Hela cells were stimulated with either 100 nM substance P or 0.5 μM TPA for 3 minutes. Following fixation in 4 % PFA and permeablizing with saponin, the cells were stained with (A) goat anti-Epsin (Santa Cruz Biotechnology Inc.), rabbit anti-Eps15 (C-20, Santa Cruz Biotechnology Inc.) and (B) mouse anti-Dab2 (BD Transduction Laboratories). For each experiment, stimulated and unstimulated cells were also stained with anti-NbC to control for the efficacy of stimulation (bottom panels in A and B). Primary antibodies were visualized using either Al488- or Cy3-labelled secondary antibodies. Size bars indicate 10μm.

• Movie 1 - Nbp66 stimulated with TPA

• Movie 2 - Numb PTBi stimulated with TPA

• Movie 3 - Numb delta218-366 stimulated with TPA

• Movie 4 - Nb p72 stimulated with SP

• Movie 5 - Numb PTBi stimulated with SP

• Movie 6 - Numb delta218-366 stimulated with SP