Supplemental Material

This article contains the following supporting material:

• Figure 1 - EM of wild type and unc-96 mutant muscle.
  EM images of cross sections through body wall muscle from wild type (wt) and all three unc-96 mutant alleles (r291, su151, and sf18). The white arrow shown in the wt image is pointing to a dense body. The black arrow is pointing to an M-line. All images were captured at the same magnification.

• Figure 2 - unc-96 and unc-15 show an enhanced phenotype as trans-heterozygotes.
  Polarized light microscopy of several body wall muscle cells from adults worms with the indicated genotypes. unc-1(e538) was used as a marker to follow the presence of unc-96(sf18) using a dissecting microscope. unc-1(e538) as heterozygote or homozygote has no effect on the polarized light phenotype of unc-15(e1215) / +. Although unc-96(sf18) / + has a normal muscle structure by polarized light, and unc-15(e1215) / + has only a mildly disorganized muscle structure by polarized light, the compound heterozygote, unc-15(e1215) / + ; unc-96(sf18) / +, displays an enhanced disorganization of muscle structure.