Supplemental Material

This article contains the following supporting material:

• Figure 1 - Sequence of ngf-1
  The ngf-1 sequence determined from cosmid G5:H10 (GenBank accession number DQ431713) corresponds to nucleotides 1,590,609 - 1,593,868 of contig 7.2 of the Neurospora crassa genome sequence (http://www.broad.mit.edu/annotation/fungi/neurospora_crassa_7/). The NGF-1 amino acid sequence predicted by the Broad Institute is shown below the DNA sequence. The HAT domain (residues 107-195) is highlighted in yellow and the bromodomain (residues 290-377) is highlighted in blue. RIP-induced C:G to T:A transition mutations of the ngf-1RIP1 allele (GenBank accession number DQ431714) are highlighted in grey. Changes in the predicted peptide sequence of NGF-1RIP1 are highlighted in grey below the wild type protein sequence and predicted stop codons are highlighted in red.