Supplemental Material

This article contains the following supporting material:

• Supplementary Figure S1 - A) Phenotypes associated with active Cdc42 (L61), Rac1 (L61) and RhoA (L63) expression in embryo. Top panels show external animal pole views and middle panels, sections through the lesions. Typical examples of resulting embryos are shown. An example of activated RhoA rescue of Epp phenotype is also shown. B) Epp RNA was co-injected with the indicated dominant negative GTPase RNA (ratio 1:1) or Epp was injected singly at the two-cell stage. Typical examples of resulting embryos are shown.
• Supplementary Figure S2 - A) Ephexin rescues xPAK1-GBD induced loss-of-adhesion. xPAK1-GBD 61-123 RNA was injected alone (200 pg) or co-injected with Ephexin mRNA (2 ng) at the two-cell stage. Top panels show animal pole views, and bottom panels show sections through the lesions. B) Quantitation of loss-of-adhesion penetrance. xPAK1-GBD and Ephexin ratios were maintained at 1:5 and 1:10, as in D). The total number of embryos analyzed is shown above each column.