Supplemental Material

This article contains the following supporting material:

• Movie 6A - Dynamics of GFP-CVAK104 in COS-7 cells. The movie shows highly motile GFP-CVAK104 labeled vesicles. The series of frames shown in the movie corresponds to 128 sec real time acquisition. See also figure 6A.

• Movie 6B - Colocalization of GFP-CVAK104 and RFP-Clathrin light chain on moving structures in living COS-7 cells. The series of frames shown in the movie corresponds to 50 sec real time acquisition. See also figure 6B.

• Movie 6C - Colocalization of GFP-CVAK104 and endocytosed Texas-Red labeled transferrin in living COS-7 cells. The series of frames shown in the movie corresponds to 122 sec real time acquisition. See also figure 6C.

• Figure 1 - Spatial relationship of CVAK104 and EEA1, respectively, with transferrin (Tf)-laden endosomes in COS-7 cells. Cells were allowed to take up pre-bound Texas-Red labeled Tf for 5 min. After fixation cells were stained either for endogenous CVAK104 or EEA1. Scale bars represent 10 μm.