Supplemental Material

This article contains the following supporting material:

• Supplemental Figure 1 - Ectopic expression from the MET3 promoter of either BEM3 or RGA2 rescues the characteristic phenotypes of the bem3Δ/Δ rga2Δ/Δ mutant. When growing under pseudohyphal conditions bem3Δ/Δ rga2Δ/Δ buds are elongated and lack constrictions at the mother cell neck. Expression of either BEM3 or RGA2 from theMET3 promoter restores constrictions to the bud neck and results in shorter and broader buds. BEM3 or RGA2 was reintroduced into the bem3Δ/Δ rga2Δ/Δ strain under the control of the MET3 promoter strain using the pCaEXP vector which integrates at the RP10 locus and uses URA3 as a selectable marker. (A-C) Stationary phase cells of the indicated genotype were reinoculated into SD medium of pH 6.0 and incubated at 36ºC for 100 minutes before samples were removed and fixed in 2.5% formaldehyde for 10 minutes. DIC images of each strain are shown. Scale bars: 5μm. D: Quantification shows the dimensions of the first pseudohyphal buds (n=50). Error bars are 95% confidence limits. Note absolute measurements of length and width are different from the values in Tables 2 and 3 due to the different growth medium used.
• Supplemental figure 2 - Localisation of Rdi1-YFP in hyphae, yeast and pseudohyphae. Rdi1-YFP shows no preferential localisation in any morphological state.
• Supplemental figure 3 - Alignment of fungal and human Rho Guanine-Dissociation inhibitors. Genes shown are the best match to a Blast search of GenBank with the C. albicans sequence. They were aligned using ClustalW multiple alignment program using default parameters available at the Pole Bioinformatique Lyonais website (http://npsa-pbil.ibcp.fr/cgi-bin/npsa_automat.pl?page=npsa_clustalw.html). Genbank accession numbers are as follows: C. albicans XP_723032.1; S.cerevisiae: NP_010146.1, C.glabrata: XP_448107; A.gossypii: NP_984194.1; K.lactis: XP_455321.1: A.fumagatus: XP_753511.1; A.nidulans: XP_657767; H.sapiens: CAA45344.1
• Supplemental Table 1