Supplemental Material

This article contains the following supporting material:

• Supplemental Figure 1 - Supplemental Figure 1: The eyes of ubc9.1 morphants display widespread apoptosis, while compromised mitosis is restricted to the ciliary marginal zones

  Dorsal view on the eyes at 48 hpf, anterior to the top, medial to the right of (A,C) ubc9.1 5mm MO injected and (B,D) ubc9.1 MO injected embryos. (A,B) Optical cross section of eyes stained with TUNEL to detect apoptotic cells. ubc9.1 morphants eyes display increased numbers of apoptotic cells, which is most prominent in the ganglion cell layer close to the lense (B,D). (C,D) Horizontal sections through eyes of embryos stained for pH3; circled regions mark the ciliary marginal zones (CMZ; compare with Figure 1N). Arrowheads mark dead cell bodies in the ganglion cell layer. Note the increased size of retinal cells in the CMZ of the morphant (D). (E) Quantification of total numbers of retinal cells per section (left panel) and the percentage of pH3-positive retinal cells (right panel). In ubc9.1 morphant embryos the number of retinal cells per section is significantly reduced (* p=4e^-6 as calculated with Student’s T-test), whereas the percentage of pH3-positive cells per section is unaltered. Error bars indicate the standard deviation (n=3). (f) Quantification of total cell numbers (left panel) and the percentage of pH3-positive cells (right panel) within the CMZ, only. ubc9.1 morphant embryos display a moderate reduction in the number of CMZ cells per section, while the percentage of pH3-positive CMZ cells per section is strongly reduced (* p=3.7e^-4 as calculated with Student’s T-test).