Supplemental Material

This article contains the following supporting material:

• Figure S1 - Supplementary Figure S1. Identification of the ~140-kDa ALG-2-binding protein by mass spectrometry (A) The peptide mass finger printing analysis chart of the ~140-kDa protein. The amino acid sequence and the positions of representative trypsin-digested fragments of Sec31A detected by mass spectrometry are indicated. (B) The ~140-kDa protein was identified as Sec31A with a MASCOT score of 177. The cross-hatched area defines the low-significance region of the MASCOT diagram.

• Figure S2 - Supplementary Figure S2. Subcellular localization of p125 in cells depleted of ALG-2 or treated with BAPTA-AM (A and A’) HeLa cells were transfected with the ALG-2 siRNA vector (A-siRNA1), and double-stained with anti-p125 (A) and anti-ALG-2 (A’) antibodies. Asterisks and arrowheads indicate ALG-2-depleted and undepleted cells, respectively. (B-C”) HeLa cells were treated without (B-B”) or with (C-C”) BAPTA-AM, and double-stained with anti-ALG-2 (B and C) and anti-p125 (B’ and C’) antibodies. B” and C” are merged images. N indicates nuclei. Bars, 20 μm.