Capping Protein and the Arp2/3 Complex Regulate Nonbundle Actin Filament Assembly to Indirectly Control Actin Bundle Positioning during Drosophila melanogaster Bristle Development
Mol. Biol. Cell Frank et al.
17: 3930
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The capping protein mutant bristle phenotype is suppressed by a reduction in function of arpc4 and arpc5, but enhanced by mutations in arpc1.
Recombinant lines bearing the cpb6.15 allele with deficiencies that remove arpc5 and arpc4 Df(2L)Exel6008 and Df(2L)Exel6015 respectively (Parks et al., 2004) were generated. The number of severely defective thoracic macrochaetes per fly was counted and data from two indendent experiments was combined. P values indicate the statistical significance of the differences between the indicated genotypes and cpbF19/cpb6.15. Numbers of bristles scored for each genotype were as follows: cpbF19/cpb6.15, 429; cpbF19 Df(Arpc5)/cpb6.15 +, 385; cpbF19 Df(Arpc4)/cpb6.15 +, 165; cpbF19 arpc1CH60/cpb6.15 +, 55; cpbF19 arpc1Q25st/cpb6.15 +, 55. Reference Parks, A.L., Cook, K.R., Belvin, M., Dompe, N.A., Fawcett, R., Huppert, K., Tan, L.R., Winter, C.G., Bogart, K.P., Deal, J.E., Deal-Herr, M.E., Grant, D., Marcinko, M., Miyazaki, W.Y., Robertson, S., Shaw, K.J., Tabios, M., Vysotskaia, V., Zhao, L., Andrade, R.S., Edgar, K.A., Howie, E., Killpack, K., Milash, B., Norton, A., Thao, D., Whittaker, K., Winner, M.A., Friedman, L., Margolis, J., Singer, M.A., Kopczynski, C., Curtis, D., Kaufman, T.C., Plowman, G.D., Duyk, G., and Francis-Lang, H.L. (2004). Systematic generation of high-resolution deletion coverage of the Drosophila melanogaster genome. Nat Genet 36, 288-292.