Supplemental Material

This article contains the following supporting material:

• Movie01 - basic motility of vegetative HPS400 cells expressing empty control vector
• Movie02 - basic motility of vegetative HPS400 cells expressing ScarΔ96GFP
• Movie03 - basic motility of vegetative Scar null cells expressing empty control vector
• Movie04 - basic motility of vegetative Scar null cells expressing ScarΔ96GFP
• Movie05 - motility and chemotaxis of developed HPS400 cells expressing empty vector
• Movie06 - motility and chemotaxis of developed Scar null cells expressing empty vector
• Movie07 - motility and chemotaxis of developed Scar null cells expressing full length Scar-GFP
• Movie08 - motility and chemotaxis of developed Scar null cells expressing ScarΔ96GFP
• Movie09 - motility and chemotaxis of developed HPS400 cells expressing ScarΔ96GFP
• Figure S1 - Expression and cell morphology of Scar null cells expressing full length Scar-GFP and Scar PWA-GFP. (A) Western blot of Scar null cells expressing full length Scar (1), scar null controls (2) or parental controls (3) using an anti-Scar antibody. Full length Scar-GFP runs ~100kDa on SDSPAGE. (B) Same samples blotted with an anti-GFP antibody. (C) Cell morphology of scar null cells expressing full length Scar-GFP. (D) Cell morphology of scar null cells expressing the C-terminal PWA fragment of Scar fused with GFP.