Supplemental Material

This article contains the following supporting material:

• Movie1 - Motility and fission of a wild type mouse liver early endocytic vesicle on a microtubule.
  In this representative study, a fluorescent early endocytic vesicle prepared from a wild type mouse liver is seen bound to a long fluorescent microtubule. Following addition of 50 μM ATP, this vesicle undergoes fission, resulting in two daughter vesicles that move in opposite directions on the microtubule. Original width: 27.23 μm; total real time: 40 sec (1 second interval); displayed at 7 frames/second.
• Movie2 - Minus-end directed movement on a microtubule of a Kifc2 knockout mouse liver early endocytic vesicle.
  In this representative study, a polarity marked microtubule with a short bright fluorescent minus end, on the left, and a long bright fluorescent plus end, on the right, is seen. A fluorescent early endocytic vesicle prepared from a Kifc2 knockout mouse liver is bound to the plus end of this microtubule. Following addition of 50 μM ATP, this vesicle moves towards the minus end. Original width: 41.06 μm; total real time: 35 sec (1 second interval); displayed at 7 frames/second.