Supplemental Materials

This article contains the following supporting material:

• Figure S2 mov - Live imaging of the Golgi apparatus from G2 through M-phase. Synchronized HeLa cells stably expressing GalNAcT2-GFP were imaged about 7 h post release by confocal microscopy at 37°C every 2 min (10 Z-axis slices per frame). Analysis of multiple movies is presented in Fig. 5
• Figure S3 avi - Live imaging of GalNAcT2-GFP fluorescence recovery in S-phase cells. Thymidine-arrested HeLa cells stably expressing GalNAcT2-GFP were imaged by epifluorescence microscopy at 37°C every 3 seconds after bleaching a specific region using a single laser pulse. Analysis of multiple movies is presented in Fig. 6.
• Figure S4 avi - Live imaging of GalNAcT2-GFP fluorescence recovery in late G2-phase cells. Olomoucine-arrested HeLa cells stably expressing GalNAcT2-GFP were imaged by epifluorescence microscopy at 37°C every 3 seconds after bleaching a specific region using a single laser pulse. Analysis of multiple movies is presented in Fig. 6.
• Figure S1 tif - GM130 depletion suppresses the MEK G2/M requirement. A. The mitotic index of GM130 siRNA transfected cells was determined at time points following release in the presence of U0126 or 0.1% DMSO carrier added at the 2 h time point. Note that in contrast to control transfected cells (e.g. Fig. 2) UO126 addition caused no G2/M delay. B. Also shown is the RMSD comparison of the deviation between mitotic progression curves as indicated. Statistical differences reflecting the delay were evident for Control + U0126 (C+U) versus GM130 knockdown (GM) and for control + U0126 (C+U) versus GM130 + U0126 (GM+U) but this was significantly reduced for the comparison of GM130 knockdown (GM) versus GM130 knockdown +U0126 (GM+U).