Supplemental Material

This article contains the following supporting material:

• Supplemental Figure 1 - The ATG26 gene is not required for selective degradation of oleate- and amine-induced peroxisomes in Y. lipolytica, but increases its efficiency in P. pastoris. Y. lipolytica WT (H222), atg26 (N155) and trs85-2 (Ain19) cells were induced in (A) oleate/ethylamine or (B) (-C)/ethylamine medium and transferred to glucose/(-N) medium to induce pexophagy. P. pastoris WT (GS200), Δ atg26 (Pdg3D) and pep4 prb1 (SMD1163) cells were induced in (C) oleate/ethylamine or (D) (-C)/ethylamine medium and transferred to glucose/(-N) medium to induce pexophagy. At the indicated time-points the culture samples were collected and processed for immunoblotting with antibodies against peroxisomal THI or AOX and mitochondrial Hsp60 proteins as described in Materials and Methods.
• Supplemental Figure 2 - The size of methanol-induced peroxisomes is much larger than that of oleate- and amine-induced peroxisomes. P. pastoris WT (GS115) cells were induced in methanol/ammonia, oleate/ammonia or (-C)/methylamine medium for 15 h and processed for electron microscopy as described above. P, peroxisome; V, vacuole. Bar, 0.5 Δm.