Supplemental Material

This article contains the following supporting material:

• Supplemental Figure 1 - Drug treatments do not perturb the sorting of content proteins to secretory granules. PHM-GFP localization was evaluated with respect to lysosomal associated membrane protein (LAMP-1), a lysosomal marker protein. Under steady state conditions (A) as well as in cells that have recovered from the hormone depletion/cycloheximide paradigm (B), PHM-GFP is localized to puncta distinct from those containing LAMP-1. Granules containing PHM-GFP are smaller in diameter than lysosomes visualized by LAMP-1 immunostaining. This segregation into vesicles of different size is maintained following both ammonium chloride (C) and lovastatin (D) treatment.