Supplemental Material

This article contains the following supporting material:

• Supplemental Figure 1 - Localization of apical and lateral marker proteins. (a) Z-section view of the immunostaining of Gp135 (red) and E-cadherin (green) in the MDCK cells and PALS1 KD#1 cells. (b) Z-section view of the F-actin staining in the MDCK cells and PALS1 KD#1 cells.
• Supplemental Figure 2 - Localization of PATJ in the PALS1 rescue cell lines. PALS1 KD cells expressing the empty vector, PALS1 wild type and various PALS1 mutants were fixed 6 h after calcium switch, and the localization of PATJ was revealed by immunostaining.