Supplemental Materials

This article contains the following supporting material:

• Oligos
• Movie01
• Movie02
• Movie03
• Movie04
• Movie05
• Movie06
• Supplemental Figure 1 - Both SSG-GFP and SGS-GFP remain in the GA upon monensin treatment together with giantin (red).
• Supplemental Figure 2 - Both GGS-GFP and GSG-GFP are localized to swollen vesicles in monensin treated cells, whereas giantin (red) remains in the GA.
• Supplemental Figure 3 - GGS-GFP (green) co-localizes with Mitotracker (red) in mitochondria, but shows additional perinuclear Golgi staining.
• Supplemental Figure 4 - Both galT-S9,11A-GFP and galT-S18A-GFP are localized to swollen vesicles in monensin treated cells, whereas giantin (red) remains in the GA.
• Supplemental Figure 5 - Both galT-S9,11D-GFP and galT-S18D-GFP are localized to swollen vesicles in monensin treated cells, whereas giantin (red) remains in the GA.