Supplemental Material

This article contains the following supporting material:

• Supplemental Figure 1 - Supplementary Figure 1. SIR3 overexpression in strains containing pJR104. Transcription of SIR3 mRNA was monitored by quantitative real-time PCR in strains used for experiments shown in Figure 5 and Table 5. SIR3 mRNA levels relative to an internal control, SCR1, was determined for each cell line and then expressed relative to that observed in SIR2 SIR3 cells containing wild-type histones H3 and H4 plus the vector YEp24, which has been set to 1. Data was calculated as follows; 2^{(SIR3 CT - SCR1 CT)SIR2 SIR3 H3 H4 plus Vector - (SIR3 CT - SCR1 CT)a}, where a = the indicated genotype, n = 1.
• Supplemental Tables