Supplemental Material

This article contains the following supporting material:

• Supplementary Figure S1 - Reference pattern of DNA replication for human primary normal keratinocytes.
  Each panel shows the frequency distribution of a parameter of DNA replication for every single donor sample (donor 1 in SFM + feeders, pink; donor 1 in SFM - feeders, blue; donor 2 in SFM - feeders, red; donor 3 in green + feeders, green; donor 3 in SFM + feeders, orange; donor 3 in SFM - feeders, cyan), and the total curve (black) obtained by pooling all the data. A. Ratio between the fork speed during the second (CldU) and the first (IdU) pulse, B. Total fork speed (kb/min), C. Ratio between the right and the left outgoing fork speed, D. Ratio between the right and the left incoming fork speed, E. Inter-origin distance (kb) and F. Replicon size (kb).
• Supplementary Figure S2 - Incorporation during fork progression.
  Green segments from type 1 signals and red segments from type 1 and 2 signals (Fig. 1A) were plotted against each other. At the top of the scatter diagrams, a schematic representation of the labeling order is shown, with black lines indicating the fluorescent tracks from individual forks that were measured and plotted against each other. A. and C. Two independent assays were done on HeLa cells, where a first 30 min pulse with IdU was followed by a second 30 min pulse with CldU. For each of them the CldU fork velocities were plotted against the IdU fork velocities (R=0.43; P<0.001, N=98). B. The nucleotide analogues were added in the inverted order, i.e. 30 min CldU followed by 30 min IdU (R=0.34, P<0.001, N=88).
• Supplementary Materials