Supplemental Material

This article contains the following supporting material:

• Supplemental Figure 1 - Non-random proximity of SCs and capillaries in human skeletal muscle
  Graphic expression of univariate (red curves) and bivariate (blue curves) point pattern analyses obtained by L(r) transformation of Ripleys K function in human deltoid muscle (n=4, age range 20-33 years). Values above the upper limit of the envelope (in between black curves) of complete spatial randomness built using the Monte Carlo method indicate clustering whereas values below its lower limit indicate regularity:
  - univariate analysis of all objects taken together, including myonuclei, SCs, and capillaries (left graph, red curve) shows both point clustering (0 to 18 μm) and regularity (25 to 50 μm). Univariate analyses of myonuclei and capillaries studied alone (right graphs, red curves) show no clustering whereas regularity imposed by the myofiber cytoplasmic area is still observed.
  - bivariate analysis of both SCs vs capillaries and myonuclei vs capillaries (left graphs, blue curves) indicate clustering of both SCs and myonuclei with capillaries, a finding not observed with virtual points introduced at random along sarcolemma (VSPs vs capillaries, right graph, blue curve). All graphs were obtained from cross-sections double-immunostained for NCAM/CD56 and CD31. (Mn means myonuclei, caps means capillaries, SCs mean satellite cells, VSPs mean virtual sarcolemmal objects).
• Supplemental Materials