Supplemental Material

This article contains the following supporting material:

• Supplemental Figure 1 - (A) Diagrammatic representation of the genetic interactions between spc7⁺ and nuf2⁺ and mis12⁺. (B) Serial dilution patch tests (10⁴ to 10¹ cells) of spc7-23, mis12-537 and a spc7-23 mis12-537 double mutant grown on YE5S for 4 (25°C) or 3 (28°C) days. (C) and (D) Serial dilution patch tests (10⁴ to 10¹ cells) of nuf2-1 and mis12-537 strains overexpressing spc7⁺ or spc7-23 cells expressing extra mis12⁺. Transformants were grown under selective conditions at the indicated temperatures for 5 to 7 days. v indicates vector control; spc7⁺ denotes the presence of wild-type spc7⁺ driven by the nmt41 promoter under derepressed conditions.
• Supplemental Figure 2 - Chromosome segregation and spindle phenotypes observed in spc7-30 and spc7-30nmt81-GFP-atb2 strains incubated at 34°C. Spindle phenotypes from left to right: wild-type anaphase spindle, anaphase spindles with a thinly staining midzone, disintegrating/broken anaphase spindles, bent spindles and unequally stained spindles. N/strain= 100.