Supplemental Material

This article contains the following supporting material:

• Video1 - Actin dynamics in control myoblasts.
  Isolated C2C12 myoblasts, which have been transfected with mRFP-actin, were plated onto anti-Fc-antibody coated dishes and actin dynamics was observed 24 hours after plating. Images were captured every 5s for 10 min and were deconvolved using the Huygens software. Deconvolved stacks were visualized as QuickTime movies (Sorenson codec was used for compression, 8 frames per second). Bar: 10μm
• Video2 - Actin dynamics on polylysine substrate
  Isolated, mRFP-actin transfected, C2C12 myoblasts were plated onto a polylysine coated dish for 24h and actin dynamics was observed, as described for video 1. This video shows the formation of highly dynamic lamellipodia.
• Video3 - M-cadherin activation induces lamellipodia formation.
  Isolated mRFP-actin transfected C2C12 myoblasts were plated onto M-cad-Fc coated dish for 24h and actin dynamics was observed as described for video 1. This video shows the formation of highly dynamic lamellipodia.
• Video4 - N-cadherin activation induces stress fibres formation.
  Isolated, mRFP-actin transfected, C2C12 myoblasts were plated onto an N-cad-Fc coated dish for 24h and actin dynamics was observed, as described for video 1. This video shows the formation of stress fibres.
• Accompanying Document 1
• Accompanying Document 2
• Accompanying Document 3