Supplemental Materials

This article contains the following supporting material:

• Supplemental Video 1 - Reconstruction of a z-sectioned, 3D-deconvolved wild type cell expressing GFP-enlazin. Each step was 0.38 μm and the exposure time was 35-ms. Every step is a rotation of 30° about the Y-axis.
• Supplemental Video 2 - Total internal reflection fluorescence movie of a wild type cell expressing GFP-enlazin. Frames were collected at 1-s intervals.
• Supplemental Video 3 - Z-stack of a z-sectioned, 3D-deconvolved wild type mitotic cell expressing GFP-enlazin. Each step was 0.38 μm and the exposure time was 35-ms. The RFP-α-tubulin image that accompanies the stack is found in Fig. 1H.
• Supplemental Video 4 - Z-stack of a z-sectioned, 3D-deconvolved myoII mutant mitotic cell expressing GFP-enlazin. Each step was 0.38 μm and the exposure time was 35-ms. The RFP-α-tubulin image that accompanies the stack is found in Fig. 1I.
• Supplemental Video 5 - Wild type control (carrying pLD1 empty vector) cell undergoing cytokinesis. Images were collected with a 20-ms exposure every 2-s.
• Supplemental Video 6 - Wild type: enlhp cell undergoing cytokinesis. Images were collected with a 20-ms exposure every 2-s.
• Supplemental Video 7 - A myoII:GFP-α-tubulin, pDRH-Hyg^R control (carrying empty pLD1 vector) undergoing cytokinesis. Movie shows the DIC movie, which accompanies Figure 5B. DIC images were collected with a 20 ms exposure every 10 s.
• Supplemental Video 8 - A myoII:GFP-α-tubulin, pDRH-Hyg^R; enlhp, undergoing cytokinesis. Movie shows the DIC movie, which accompanies Figure 5C. DIC images were collected with a 20 ms exposure every 10 s.
• Supplemental Video 9 - A cortI¹¹⁵¹:cortI (rescued) cell undergoing cytokinesis. Images were collected with a 20-ms exposure every 2-s.
• Supplemental Video 10 - A cortI¹¹⁵¹ control (carrying pLD1 empty vector) cell undergoing cytokinesis. Images were collected with a 20-ms exposure every 2-s.
• Supplemental Video 11 - A cortI¹¹⁵¹:enl-tr cell undergoing cytokinesis. Images were collected with a 20-ms exposure every 2-s.
• Supplemental Video 12 - A cortI¹¹⁵¹:enlhp cell undergoing cytokinesis. Images were collected with a 20-ms exposure every 2-s.