Supplemental Material

This article contains the following supporting material:

• Supplemental Movie 1 - Cytoplasmic mixing occurs concomitant with mating pair lysis. Lysis of prm1Δ x prm1Δ mating pairs in media lacking Ca²⁺ was imaged at one frame per every 5 seconds. The prm1Δ MATα cell is marked by cytoplasmic GFP expression. This movie plays 10 frames/sec.
• Supplemental Figure 1 - Kinetics of fusion and lysis initiation of prm1Δ fus1Δ x prm1Δ fus1Δ mating pairs. Time lapse microscopy of a prm1Δ fus1Δ x prm1 Δfus1Δ mating mixture was performed on SDC agar slips. Fusion and lysis events were binned into five minute time windows and normalized to indicate the percentage of the mating pair population that would undergo the indicated event within these time windows.