Supplemental Materials

This article contains the following supporting material:

• Movie 1 - Localization of Pan1-GFP (green) and Abp1-RFP (red) in wild type, sla2ΔCC, and sla2ΔCC pan1ΔWA cells. Interval between frames is 1 s.
• Figure 1 - Pan1p activity is important for initiating actin assembly and endocytic internalization. (A-D) Left panels are single frames from a two-color movie showing Abp1-RFP (red) and Sla1-GFP (green) in wild-type (A), pan1Δ855 (B), las17ΔWCA (C), or pan1Δ855 las17ΔWCA (D) cells. Right panels are time series of single patches. Times to acquire one image pair are 2.2 s (A-C) and 3.5 s (D). The average lifetimes were obtained from at least 50 patches. (E) Single frames from live-cell movies showing localization of Sla1-GFP in wild-type (top) or pan1Δ855 las17ΔWCA (bottom) cells with corresponding kymographs on right. Lines in images (left panels) mark where the kymograph was generated. Each kymograph shows dynamics of Sla1p patches on opposite sides of a cell for 3 min. Kymographs of time-lapse images collected at 1 s intervals.