Supplemental Material

This article contains the following supporting material:

• Supplemental Figure 1 - AP-2 localization is unaffected by AAK1L CBD2 overexpression. tTA HeLa cells were infected with control tTA (A), full-length AAK1s (B), AAKL (C), or AAK1L CBD2 (D) encoded adenoviruses. Following a 24 hr infection, cells were processed for immunolocalization AP-2 as described for clathrin (Figure 3). AP-2 was detected with the monoclonal antibody AP.6. Non-overexpressing cells are indicated with an asterisk. scale bar, 20 µm