Supplemental Material

This article contains the following supporting material:

• Figure S1 - PCR analyses of transcription expression of major satellites in Np95 deleted cells. Relative RNA levels for major satellites and GAPDH was determined by RT-PCR analyses on cDNA derived from total RNA obtained from Np95 depleted (Np95 RNAi) and control cells (Ctrl RNAi). The picture shows the results of 18, 20, 22, 24 cycles of amplification of the major satellite to show that 18 cycles is in a linear range of amplification. The negative digital image of agarose gel analysis are shown.