Supplemental Material

This article contains the following supporting material:

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• Supplemental Figure 1 - HL-60 cell differentiation is not affected by calpain 2 expression. Mac-1 and ICAM-1 expression was determined in HL-60 cells that express control, wild type calpain 2, or calpain 2 protease dead GFP-fusion constructs. (A) Undifferentiated (solid lines) and DMSO-differentiated HL-60 cells (dashed lines) that express either control GFP, calpain 2-GFP, or calpain 2 PD-GFP fusion constructs were stained with either α-Mac-1 (grey) or α-ICAM-1 (black) antibody and analyzed by FACS as described in materials and methods. (B) Mean fluorescence intensities from one representative experiment are shown.