Supplemental Material

This article contains the following supporting material:

• Figure S1 - MRI of mice as indicated in Methods. Data presented represent a representative experiment done with knockout and WT animals. c, cerebellum; e, eye; n, nose; v, ventricle. RMI reveals tissues with high water content (bright areas) such as the brain ventricles, eye balls, ears, tooth medulla, heart, bladder, etc. The increase in the size and brightness of the brain ventricles is clearly observed in the frontal (top) and horizontal (bottom) RMI images of heads of Vav2^–/– mice when these are compared to identical RMI layers obtained from WT animals (n=6 in each group).
• Figure S2 - Vasopressin and aldosterone levels in propranolol–treated animals. (A,B) Effect of propranolol on the plasma levels of vasopressin (A, n=5) and aldosterone (B, n=5) in mice of the indicated genotypes. U, untreated; P, propranolol–treated animals.