Supplemental Material

This article contains the following supporting material:

• GFP-ARNO and Arf1-RFP in Cos cells
• GFP-EFA6 and Arf1-RFP in Cos cells
• Supplemental Figure 1 - Quantification of PM localization of GEF constructs.
  The percentage of cells that exhibited predominant membrane localization of the GEF constructs with little obvious cytosolic staining was scored for a hundred cells in three independent experiments. Gray bars represent the construct transfected by itself and black bars represent co-transfection of the construct with Arf6. Shown is the average of three experiments ± 1 SD.