Supplemental Material

This article contains the following supporting material:

• Figure S1A
• Figure S1B - Wild type (A) and icmA^- cells (B) were washed free of bacteria, pulsed with cAMP and plated in an under agar chemotaxis assay.
• Figure S2A
• Figure S2B - Wild type (A) and icmA^- cells (B) expressing GFP-myosin II were pulsed with cAMP, plated in an under-agar motility assay and viewed with epifluorescence optics.
• Figure S3A
• Figure S3B - Wild type (A) and icmA^- (B) cells expressing N-term PI3K-GFP were pulsed with cAMP and plated on glass-bottomed petri dishes. A burst of cAMP was applied from a nearby pipette connected to a pulse controller. Relocalization of cAMP occurs in both cell types with 6 sec of treatment with cAMP.
• Figure S4A
• Figure S4B - Cells expressing GFP-PhdA were pulsed with cAMP and the subjected to bursts of cAMP from a nearby micropipette. Protein relocalization in wild type cells is seen within 6 sec of application of cAMP (A). Most icmA^- cells fail to respond (B).
• Figure S5 - Loading controls: EtBr stained gels corresponding to the Northern blots in Fig 2. Top, middle, and bottom panels correspond to the top, middle, and bottom panels of Fig 2B, respectively.