Supplemental Material

This article contains the following supporting material:

• Supplemental Figure 1 - MPM-2 foci depend on the cell cycle. w¹¹¹⁸ syncytial blastoderm embryos were stained with α-PH3 (left panels in blue), α-P-Tyr (left panels in red), and MPM-2 (middle panels, green in merge). Shown are cycle 12 nuclei in (A) prophase, (B) metaphase, and (C) anaphase. (D) Cycle 13 interphase nuclei. Bar: 10 μm.
• Supplemental Figure 2 - MPM-2 foci are not dependent on Lsm11. Dmel-2 cells treated with either (A) control dsRNA or (B) Lsm11 dsRNA were stained with MPM-2 (left panels in green), α-Lsm11 (middle panels in red), and DAPI (right panels in blue). Arrow in (A) indicates a cell with a single HLB. Arrow and arrowhead in (B) indicates cells with two or three, respectively, MPM-2 foci and no Lsm11 foci. Bar:10 μm.
• Supplemental Figure 3 - The histone locus body first forms and co-localizes with MPM-2 foci during nuclear cycle 11. Syncytial blastoderm embryos with a maternally provided supply of YFP-Lsm11 were stained with MPM-2 (red) and α-GFP (YFP-Lsm11; green). Interphase of nuclear cycles 9, 10, 11 and 13 are shown, as indicated on the left. Bar: 10 μm.