Supplemental Material

This article contains the following supporting material:

• Supplemental Figure 1 - Effect of the TG repeat length on Cdc13 association with DNA ends.
  HO-CA (TG(-)), TG₁₁-HO-CA (TG₁₁) and TG-HO-CA (TG₈₁) cells expressing Cdc13-myc were analyzed by ChIP assay as in Fig. 3A.
• Supplemental Figure 2 - Effect of Cdc13-Est1 expression on de novo telomere synthesis at the TG11 end
  TG-HO-CA (TG₈₁) or TG₁₁-HO-CA (TG₁₁) cells carrying the GAL-HO plasmid were transformed with YCp-CDC13-EST1 (pCDC13-EST1) or the control vector, and plated out on galactose medium selectable for the plasmid. Individual colonies were isolated and cultured in medium selectable for the plasmids, and subjected to Southern blot analysis using the probe A as in Fig. 1C. All the analyzed cells were found to add telomeres at the DSB end. Telomeres in some TG₁₁-HO-CA cells were longer than those in TG-HO-CA cells, consistent with the previous finding that expression of Cdc13-Est1 proteins elongate endogenous telomeres (Tsukamoto et al., 2001).