Supplemental Material

This article contains the following supporting material:

• Supplementary Figure 1 - (A) PFGE analysis of genomic DNA suggests that the chromosomes of three independent taz1-d rad11-D223Y rqh1-d triple mutants are not circularized. PFGE was performed as in Figure 1E. (B) The telomere length of the wild-type cells (lane 1, JY746), rad11-D223Y cells (lane 2, YO001), taz1-d rad11-D223Y rqh1-d cells (lane 3, TK025), and three independent taz1-d rqh1-d trt1-d triple-mutant cells (lane 4-6, TK062) was analyzed by Southern hybridization. Genomic DNA was digested with ApaI, fractioned by 1.0% agarose gel electrophoresis, and hybridized to telomere probe.