Supplemental Materials

This article contains the following supporting material:

• Supplemental Figure 1 - Levels of accumulation of Abl and Bcr-Abl after mis-expression. A. Immunoblot of extracts from embryos misexpressing Bcr-Abl from the indicated transgenes using e22c-GAL4, probed with anti-Bcr. BicaudalD (BicD) serves as a loading control. B. Immunoblot of extracts from embryos overexpressing wild-type Abl or kinase-dead Abl from the indicated transgenes using e22c-GAL4, probed with anti-Abl, and re-probed with anti-Arm. Peanut (Pnut) serves as a loading control. C. Extracts from wild-type (wt) or p185 misexpressing embryos were immunoprecipitated with anti-Abl antibody, and sequentially blotted with antibodies to Abl or to phosphotyrosine. Arrowhead=position of p185.
• Supplemental Figure 2 - Abl and Bcr-Abl do not affect cell polarity or microtubule localization. Embryos, stage 12 (A) or 13 (B-F), anterior left and lateral views (except E, which is a ventral view, and G, which is a dorsal view). Antigens, transgenes and GAL4 drivers indicated. Bar=20µm.
• Supplemental Table 1
• Video 1 - Wild-type embryo expressing Moesin-GFP during dorsal closure. Stills in Figure 7A. Images were collected every 10 seconds but to reduce movie size only every third frame is shown. Playback rate is 16 frames per second.
• Video 2 - Closeup of amnioserosal cells during dorsal closure in wild-type embryo expressing Moesin-GFP. Note filopodial projections. Images were collected every 15 seconds. Playback rate is 16 frames per second.
• Video 3 - Embryo expressing Moesin-GFP and misexpressing p185 ubiquitously using e22c-GAL4 during dorsal closure. Stills are in Figure 7B. Images were collected every 10 seconds but to reduce movie size only every third frame is shown. Playback rate is 16 frames per second.
• Video 4 - Closeup of amnioserosal cells during dorsal closure in embryo expressing Moesin-GFP and misexpressing p185 ubiquitously using e22c-GAL4. Note absence of filopodia and replacement by broad lamellipodia. Images were collected every 10 seconds. Playback rate is 24 frames per second.
• Video 5 - Embryo expressing Moesin-GFP and misexpressing p210 ubiquitously using e22c-GAL4 during dorsal closure. Stills are in Figure 7C. Images were collected every 10 seconds but to reduce movie size only every third frame is shown. Playback rate is 16 frames per second.
• Video 6 - Embryo expressing Moesin-GFP and overexpressing wild-type Abl ubiquitously using e22c-GAL4 during dorsal closure. Stills are in Figure 7D. Images were collected every 10 seconds but to reduce movie size only every third frame is shown. Playback rate is 16 frames per second.
• Video 7 - Wild-type embryo expressing actin-GFP in epidermal stripes during dorsal closure using en-GAL4, Stills are in Figure 8F. Images were collected every 10 seconds. Playback rate is 16 frames per second.
• Video 8 - Embryo expressing actin-GFP and misexpressing p185 in epidermal stripes during dorsal closure using en-GAL4, Stills are in Figure 8G. Images were collected every 10 seconds. Playback rate is 16 frames per second.
• Video 9 - Embryo expressing actin-GFP and overexpressing wild-type Abl in epidermal stripes during dorsal closure using en-GAL4, Stills are in Figure 8H. Images were collected every 10 seconds. Playback rate is 16 frames per second.