Supplemental Material

This article contains the following supporting material:

• Figure S1 - E-cadherin depletion delays but does not block apical surface formation
  Percentage of cells with apical gp135 staining in mock-depleted (black bars) or E-cadherin depleted (blue bars) MDCK cell monolayers as determined from 40x confocal images at different time points after Ca-switch; data (presented with standard errors) are from 3 independent experiments; a total of ~350 cells were scored in each experiment; merged confocal x-y images show gp135 (green) and ZO1 (blue) staining 3,5 and 12h after Ca-switch.