Supplemental Materials

This article contains the following supporting material:

• Figure S1. - Cortical LET-99 asymmetry does not require mex-5, mex-6 and spn-4. (A) Confocal images of PKC-3 and LET-99 double staining in wild-type and mex-5;mex-6 embryos. (B) Confocal images of LET-99 localization in spn-4 mutant embryos. (C) Quantification of whole embryo LET-99 staining intensities in wild-type, par-1, mex-5;mex-6, spn-4 and let-99 embryos. Error bars show s.e.m. Total LET-99 intensities are more than 50% lower in mex-5;mex-6 embryos (p<0.0001), compared to other backgrounds measured. The intensities for spn-4 embryos also appear lower than wild-type (p=0.051 for prophase, p=0.09 for metaphase, p= 0.10 for anaphase) but not as different as mex-5;mex-6. Scale bar: 10 μm.
• Movie 1 - DIC time-lapse movie of a wild-type one-cell embryo from pronuclear migration to telophase.
• Movie 2 - DIC time-lapse movie of a par-1 one-cell embryo from pronuclear migration to telophase.
• Movie 3 - DIC time-lapse movie of a par-4 one-cell embryo from pronuclear migration to telophase.
• Movie 4 - DIC time-lapse movie of a par-1 one-cell embryo without eggshell from pronuclear migration to telophase.
• Movie 5 - DIC time-lapse movie of a wild-type two-cell embryo with a spherical P1 cell.
• Movie 6 - DIC time-lapse movie of a par-1 two-cell embryo with a spherical P1 cell.
• Movie 7 - DIC time-lapse movie of a par-1 two-cell embryo with a spherical P1 cell and an ectopic flat side produced by the coverslip on top.
• Movie 8 - DIC time-lapse movie of a wild-type two-cell embryo with a spherical P1 cell and an ectopic flat side produced by the coverslip on top.