G protein signaling governing cell fate decisions involves opposing G subunits in Cryptococcus neoformans

Supplemental Material

This article contains the following supporting material:

• Supplemental Figure 1 - gpa2 gpa3 double mutants are fertile when crossed to gpa2 or gpa3 single mutants. Serotype A strains of the indicated genotypes were crossed on MS mating medium. The photos of the mating colonies and basidiospore chains were taken at 100x magnification after 2 weeks incubation in the dark at 25°C.
• Supplement Figure 2 - Protein alignment of fungal Gγ subunits. Amino acid sequences of fungal Gγ subunits were retrieved from GenBank and aligned with the ClustalW program. A conserved CAAX motif is identified at the C-terminus. The accession numbers of the sequences are AAX94563 (Cryptococcus neoformans), EAU88842 (Coprinopsis cinerea), Q870G5 (Lentinula edodes), ABG73390 (Aspergillus fumigatus), AAV83542 (Neurospora crassa), and NP_012619 (Saccharomyces cerevisiae).
• Supplemental Figure 3 - Overexpression of GPA2 or GPA3 inhibits mating in C. neoformans. Serotype D strains of the indicated genotypes were crossed on V8 mating medium. The edges of mating colonies were photographed at 100x magnification after 24 hour incubation in the dark at 25°C.
• Supplemental Table 1