Supplemental Materials

This article contains the following supporting material:

• Supplemental Figure 1 - Myo1B localization in the adult optic lobe and photoreceptors. A-C. Confocal imaging of cryosections of w¹¹¹⁸ adult head. Sections were co-stained for Myo1B (green) and actin (red), overlays are shown in column 3. (A) Optic lobe region. L = lamina, M = medulla, Lo = lobula, Lp = lobular plate, the arrow marks the inner chiasm. In the optic lobe, Myo1B exhibits relatively high levels of expression in the inner chiasm, although there is also significant expression in the lamina and medulla. (B) Higher magnification image of the lamina and medulla; arrow marks the outer chiasm. (C) Distal region of the eye; asterisk marks the pseudocone. (D) Section along the length of the photoreceptors. Myo1B is expressed in photoreceptors but in contrast to its colocalization with MV actin in the midgut BB, Myo1B is largely absent from the actin rich-rhabdomere MV and is concentrated at the base of MV. A - B, bar = 50 μm. C - D, bar = 25 μm.
• Supplemental Figure 2 - Histology of whole mount, serial sectioned Myo1B heterozygous third instar larva. Imaging begins at the dorsal surface and moves toward the ventral surface of the larva. Bar = 1mm.
• Supplemental Figure 3 - Histology of whole mount, serial sectioned Myo1B homozygous third instar larva. Imaging begins at dorsal surface and moves toward the ventral / lateral surface of the larva. As compared with heterozygous control, there does not appear to be any gross abnormalities of organ morphology due to the loss of Myo1B. Bar = 1mm.