Supplemental Materials

This article contains the following supporting material:

• Supplementary Figure - Characterization of Src mutants. (A) Schematic representation of all Src mutations used in this study. (B) The mutation of W118 to K mutation on the SH3 adaptor function was analyzed by using the indicated GST-fusion proteins in a in vitro pull-down assay (left panels). This mutation abolished the interaction between Src SH3 domain and its main partner, Cbl. (C,D) The kinase activity of each Src mutant was analyzed by two independent approaches: (C) 293-VnR cells were transfected with indicated Src mutants. Src IPs were blotted for phospho-SrcY⁴¹⁶ (top), a marker of Src kinase activation, then stripped and reprobed for Src (bottom). (D) 293-VnR cells were transfected with the indicated Src mutants. Src was immunoprecipitated and Src kinase activity was measured by a commercial in vitro kinase assay using a peptide as a substrate.