Supplemental Materials

This article contains the following supporting material:

• Supplemental Figure 1 - Effects of the cell permeable derivative of C3 exoenzyme on RhoA localization and cytokinesis. (A) Control and C3-loaded HT1080 cells were stained with anti-RhoA antibody after fixation with TCA; scale bars, 20 μm. (B) Failed cytokinesis in a C3-loaded HT1080 cell on a substrate without collagen coating. Numbers indicate the time in minutes after onset of anaphase; scale bar, 20 μm.
• Supplemental Figure 2 - Actin and focal adhesion remodeling induced by overexpression of mCherry-Ect2-DA. (A, B) HT1080 cells were transfected with a plasmid encoding mCherry-Ect2-DA, and the actin cytoskeleton and the focal adhesions were visualized using FITC-labeled phalloidin (A, left) and anti-vinculin antibody (B, left; clone hVIN-1, Sigma), respectively. Cells expressing mCherry-Ect2-DA was identified by mCherry fluorescence (right). (C) HT1080 cells were transfected with a plasmid encoding GFP-V12-Rac1 (Kanada, Nagasaki and Uyeda, unpublished). The actin cytoskeleton was visualized using rhodamine-labeled phalloidin (left). The expression of GFP-V12-Rac1 was confirmed by GFP fluorescence (right). Scale bars, 20 μm.
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